fulltext.study @t Gmail

Protein repellent properties of covalently attached PEG coatings on nanostructured SiO2-based interfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
10643 696 2007 9 PDF Available
Title
Protein repellent properties of covalently attached PEG coatings on nanostructured SiO2-based interfaces
Abstract

In this study, we report the systematic comparison of different poly(ethylene glycol) (PEG) self-assembled monolayers on glass with respect to their protein adsorption and cell adhesion resistance. Combining PEGylation with micellar nanolithography allowed the formation of gold nanoparticle arrays on glass and selective coverage of the free glass area by PEG. The gold nanoparticles serve as anchor points for the attachment of individual proteins and peptides such as the cell–matrix adhesion promoting cyclic RGDfK motif or the kinesin motor protein Eg5. The capability of the motor protein to bind microtubules remained unaffected by the immobilization. It was shown that the film thickness of a water swollen PEG layer is crucial to maximize the interaction between proteins and peptides with the nanostructures. Non-specific interaction between cells or microtubules and the surface was minimized. The optimum PEG layer thickness correlated with the size of gold nanoparticles which was approximately 5 nm.

Keywords
Poly(ethylene glycol) (PEG); Cell adhesion; Protein adsorption; Quartz crystal microbalance (QCM); Nanostructures; Biofunctionalization
First Page Preview
Protein repellent properties of covalently attached PEG coatings on nanostructured SiO2-based interfaces
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 32, November 2007, Pages 4739–4747
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us