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Islet-encapsulation in ultra-thin layer-by-layer membranes of poly(vinyl alcohol) anchored to poly(ethylene glycol)–lipids in the cell membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
10651 696 2007 8 PDF Available
Title
Islet-encapsulation in ultra-thin layer-by-layer membranes of poly(vinyl alcohol) anchored to poly(ethylene glycol)–lipids in the cell membrane
Abstract

The microencapsulation of islets of Langerhans (islets) in a semipermeable membrane, i.e., the creation of a bioartificial pancreas, has been studied as a safe and simple technique for islet transplantation without the need for immunosuppressive therapy. The total volume of the implant tends to increase after enclosure of the islets in the semipermeable membrane, which limits transplantation sites. Thus, ultra-thin membranes are required for clinical applications. Here, we propose a novel method to encapsulate islets in an ultra-thin membrane of poly(vinyl alcohol) (PVA) anchored to a poly(ethylene glycol) (PEG)–phospholipid conjugate bearing a maleimide group (Mal–PEG–lipids, PEG Mw: 5000) in the cell membranes of islets. When Mal–PEG–lipids were added to an islet suspension, they spontaneously formed a thin layer on cells of the outer layer of islets. The PEG–lipid layer on the islets was covered by a PVA monolayer, and the PVA membrane was further reinforced by using the layer-by-layer method with thiol/disulfide exchange reactions. No practical volume increase in islets was observed after microencapsulation by this method. In addition, encapsulation of the islet surface in PVA membranes did not impair insulin release in response to glucose stimulation.

Keywords
Bioartificial pancreas; Islets; Poly(ethylene glycol)–lipid (PEG–lipid); Poly(vinyl alcohol) (PVA); Layer-by-layer membrane
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Islet-encapsulation in ultra-thin layer-by-layer membranes of poly(vinyl alcohol) anchored to poly(ethylene glycol)–lipids in the cell membrane
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 32, November 2007, Pages 4818–4825
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us