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The influence of implantation site on the biocompatibility and survival of alginate encapsulated pig islets in rats

Paper ID Volume ID Publish Year Pages File Format Full-Text
10707 699 2006 8 PDF Available
Title
The influence of implantation site on the biocompatibility and survival of alginate encapsulated pig islets in rats
Abstract

This work investigated the impact of implantation sites on the biocompatibility of alginate encapsulated pig islets. Non-diabetic rats were implanted with adult pig islets encapsulated in alginate either intraperitoneally (IP; n=25n=25), subcutaneously (SC; n=37n=37) or under the kidney capsule (KC; n=34n=34). Capsule biocompatibility (retrieval rate, capsule diameter, degree of capsule broken and cellular overgrowth, CD68/CD3 staining) as well as islets viability and functionality were assessed until 30 days after transplantation. Implantation site did not significantly influence the biocompatibility of empty alginate capsules after transplantation (n=48n=48). Most of the empty capsules (>90%) were retrieved after harvesting and were free of cellular overgrowth until day 30 post-transplantation. Three days after implantation, no significant difference for encapsulated pig islets was observed in terms of capsule biocompatibility and islet functionality in peritoneum, KC or subcutaneously. However, between days 5 and 30 after transplantation, explanted capsules from IP demonstrated a higher degree of broken capsules (>13%) and capsules with severe cellular overgrowth (>50%, CD68+ infiltration) than capsules removed from SC and KC (p<0.05p<0.05). This was associated with a significant reduction of islet viability, insulin content and insulin secretion.In rats, the peritoneum site seems not appropriate for promoting the engraftment of encapsulated pig islets. Kidney subcapsular and subcutaneous spaces represent an interesting alternative.

Keywords
Islet; Microencapsulation; Biocompatibility; Xenotransplantation
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The influence of implantation site on the biocompatibility and survival of alginate encapsulated pig islets in rats
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 17, June 2006, Pages 3201–3208
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us