fulltext.study @t Gmail

Modified montmorillonite as vector for gene delivery

Paper ID Volume ID Publish Year Pages File Format Full-Text
10721 699 2006 6 PDF Available
Title
Modified montmorillonite as vector for gene delivery
Abstract

Currently, gene delivery systems can be divided into two parts: viral or non-viral vectors. In general, viral vectors have a higher efficiency on gene delivery. However, they may sometimes provoke mutagenesis and carcinogenesis once re-activating in human body. Lots of non-viral vectors have been developed that tried to solve the problems happened on viral vectors. Unfortunately, most of non-viral vectors showed relatively lower transfection rate. The aim of this study is to develop a non-viral vector for gene delivery system.Montmorillonite (MMT) is one of clay minerals that consist of hydrated aluminum with Si–O tetrahedrons on the bottom of the layer and Al–O(OH)2 octahedrons on the top. The inter-layer space is about 12 Å. The room is not enough to accommodate DNA for gene delivery. In the study, the cationic hexadecyltrimethylammonium (HDTMA) will be intercalated into the interlayer of MMT as a layer expander to expand the layer space for DNA accommodation. The optimal condition for the preparation of DNA–HDTMA–MMT is as follows: 1 mg of 1.5CEC HDTMA–MMT was prepared under pH value of 10.7 and with soaking time for 2 h. The DNA molecules can be protected from nuclease degradation, which can be proven by the electrophoresis analysis. DNA was successfully transfected into the nucleus of human dermal fibroblast and expressed enhanced green fluorescent protein (EGFP) gene with green fluorescence emission. The HDTMA–MMT has a great potential as a vector for gene delivery in the future.

Keywords
Composite; DNA; Gene transfer; Silicate
First Page Preview
Modified montmorillonite as vector for gene delivery
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 17, June 2006, Pages 3333–3338
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us