fulltext.study @t Gmail

Immediate production of a tubular dense collagen construct with bioinspired mechanical properties

Paper ID Volume ID Publish Year Pages File Format Full-Text
1073 74 2012 13 PDF Available
Title
Immediate production of a tubular dense collagen construct with bioinspired mechanical properties
Abstract

The intrinsic complexity of tissues and organs demands tissue engineering approaches that extend beyond planar constructs currently in clinical use. However, the engineering of cylindrical or tubular tissue constructs with a hollow lumen presents significant challenges arising from geometrical and architectural considerations required to tailor biomaterials for tissue and organ repair. Type I collagen is an ideal scaffolding material due to its outstanding biocompatibility and high processability. However, the highly hydrated nature of collagen hydrogels results in their lack of mechanical properties and instability, as well as extensive cell-mediated contraction, which must be overcome to achieve process control. Herein, tubular dense collagen constructs (TDCCs) were produced simply and rapidly (in less than 1 h) by circumferentially wrapping plastically compressed dense collagen gel sheets around a cylindrical support. The effects of collagen source, i.e. rat-tail tendon and bovine dermis-derived acid solubilized collagen, and concentration on TDCC properties were investigated through morphological, mechanical and chemical characterizations. Both tensile strength and apparent modulus correlated strongly with physiologically relevant collagen gel fibrillar densities. The clinical potential of TDCC as a tubular tissue substitute was demonstrated mechanically, through circumferential tensile properties, theoretical burst pressure, which ranged from 1225 to 1574 mm Hg, compliance values of between 8.3% to 14.2% per 100 mm Hg and suture retention strength in the range of 116–151 grams-force, which were compatible with surgical procedures. Moreover, NIH/3T3 fibroblast viability and uniform distribution within the construct wall were confirmed up to day 7 in culture. TDCCs with fibrillar densities equivalent to native tissues can be readily engineered in various dimensions with tunable morphological and mechanical properties, which can be easily handled for use as tissue models and adapted to clinical needs.

Graphical abstractA tubular dense collagen construct (TDCC) has been developed with tunable and defined geometrical, morphological and mechanical properties in less than an hour via plastic compression technique, dramatically reducing the time for scaffold manufacturing. The biologically relevant mechanical strength and compliance together with appropriate suturability, present the TDCC as an optimal candidate for tubular tissue substitution.Figure optionsDownload full-size imageDownload high-quality image (59 K)Download as PowerPoint slide

Keywords
Vascular; Plastic compression; Tube; Tissue model; Compliance
First Page Preview
Immediate production of a tubular dense collagen construct with bioinspired mechanical properties
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 8, Issue 5, May 2012, Pages 1813–1825
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us