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Towards improved artificial lungs through biocatalysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
10732 700 2007 9 PDF Available
Title
Towards improved artificial lungs through biocatalysis
Abstract

Inefficient CO2 removal due to limited diffusion represents a significant barrier in the development of artificial lungs and respiratory assist devices, which use hollow fiber membranes (HFMs) as the blood–gas interface and can require large blood-contacting membrane area. To offset the underlying diffusional challenge, “bioactive” HFMs that facilitate CO2 diffusion were prepared via covalent immobilization of carbonic anhydrase (CA), an enzyme which catalyzes the conversion of bicarbonate in blood to CO2, onto the surface of plasma-modified conventional HFMs. This study examines the impact of enzyme attachment on the diffusional properties and the rate of CO2 removal of the bioactive membranes. Plasma deposition of surface reactive hydroxyls, to which CA could be attached, did not change gas permeance of the HFMs or generate membrane defects, as determined by scanning electron microscopy, when low plasma discharge power and short exposure times were employed. Cyanogen bromide activation of the surface hydroxyls and subsequent modification with CA resulted in near monolayer enzyme coverage (88%) on the membrane. The effect of increased plasma discharge power and exposure time on enzyme loading was negligible while gas permeance studies showed enzyme attachment did not impede CO2 or O2 diffusion. Furthermore, when employed in a model respiratory assist device, the bioactive membranes improved CO2 removal rates by as much as 75% from physiological bicarbonate solutions with no enzyme leaching. These results demonstrate the potential of bioactive HFMs with immobilized CA to enhance CO2 exchange in respiratory devices.

Keywords
Artificial respiratory device; Hollow fiber membrane; Carbon dioxide; Gas exchange; Carbonic anhydrase; Enzyme immobilizationHFM, hollow fiber membrane; CA, carbonic anhydrase
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Towards improved artificial lungs through biocatalysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 20, July 2007, Pages 3131–3139
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us