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Effect of biomaterial surface properties on fibronectin–α5β1 integrin interaction and cellular attachment

Paper ID Volume ID Publish Year Pages File Format Full-Text
10782 702 2006 10 PDF Available
Title
Effect of biomaterial surface properties on fibronectin–α5β1 integrin interaction and cellular attachment
Abstract

The ability of fibronectin (Fn) to mediate cell adhesion through binding to α5β1 integrins is dependent on the conditions of its adsorption to the surface. Using a model system of alkylsilane SAMs with different functional groups (X=OH, COOH, NH2 and CH3) and an erythroleukemia cell line expressing a single integrin (α5β1), the effect of surface properties on the cellular adhesion with adsorbed Fn layers was investigated. 125I-labeled Fn, a modified biochemical cross-linking/extraction technique and a spinning disc apparatus were combined to quantify the Fn adsorption, integrin binding and adhesion strength, respectively. This methodology allows for a binding equilibrium analysis that more closely reflects cellular adhesion found in stable tissue constructs in vivo. Differences in detachment strength and integrin binding were explained in terms of changes in the adhesion constant (ψ, related to affinity) and binding efficiency of the adsorbed Fn for the α5β1 integrins (CH3≈NH2

Keywords
Cell adhesion; Fibronectin; Protein adsorption; Integrin; interface; Adhesion mechanism.
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Effect of biomaterial surface properties on fibronectin–α5β1 integrin interaction and cellular attachment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 9, March 2006, Pages 1907–1916
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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