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Attenuated alloreactivity of dendritic cells engineered with surface-modified microspheres carrying a plasmid encoding interleukin-10

Paper ID Volume ID Publish Year Pages File Format Full-Text
10802 702 2006 7 PDF Available
Title
Attenuated alloreactivity of dendritic cells engineered with surface-modified microspheres carrying a plasmid encoding interleukin-10
Abstract

In the present study, we investigated MSO10H6MSO10H6 as a carrier system to introduce a plasmid encoding murine interleukin-10 (pIL-10) to modulate alloreactivity of dendritic cells (DC). Results indicate that MSO10H6MSO10H6 formed stable and protective nano-sized particles with pIL-10. Gene-modified DC elicited weak proliferation of allogeneic CD4 and CD8 T cells in vitro. Using cell-embedded Matrigel as a surrogate graft, we also showed that DC transfected with MSO10H6MSO10H6 complexed with pIL-10 suppressed host cell infiltration in vivo. These data demonstrate that the self-assembled system of MSO10H6MSO10H6 is an effectual delivery vehicle for plasmid-based modulation of DC-dependent allogeneic T cell responses.

Keywords
Non-viral gene delivery; Dendritic cells; Microspheres; Interleukin-10; Allogeneic reactionMS, Microspheres; O10H6O10H6, O=ornithine; H=histidine; DC, Dendritic cells
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Attenuated alloreactivity of dendritic cells engineered with surface-modified microspheres carrying a plasmid encoding interleukin-10
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 9, March 2006, Pages 2076–2082
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us