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Rapid localized cell trapping on biodegradable polymers using cell surface derivatization and microfluidic networking

Paper ID Volume ID Publish Year Pages File Format Full-Text
10804 702 2006 5 PDF Available
Title
Rapid localized cell trapping on biodegradable polymers using cell surface derivatization and microfluidic networking
Abstract

Spatial control over cell attachment is essential for controlling cell behavior and engineering cell-based sensor arrays. Here we report on a patterning procedure that can be utilized on a wide range of adherent and non-adherent cell types without the need to identify the exact peptide sequence or extracellular matrix (ECM) necessary for optimal cell attachment. This is achieved by converting native sialic residues present on the surface of most cells into non-native aldehydes using a mild sodium periodate treatment. The aldehyde groups are then reacted with biotin hydrazide to produce biotinylated cells. Avidin is patterned onto the surface of a biotinylated biodegradable block copolymer, polylactide–poly(ethylene glycol)–biotin (PLA–PEG–biotin) by microfluidic networking using a PDMS stamp. The biotinylated cells then bind specifically to the patterned avidin regions. The PEG that is presented from the PLA–PEG–biotin copolymer in the regions without avidin immobilization minimizes cell binding in the non-patterned regions.

Keywords
Cell surface engineering; Polymer synthesis; Microfluidics; Soft lithography; Biotin–avidin
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Rapid localized cell trapping on biodegradable polymers using cell surface derivatization and microfluidic networking
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 9, March 2006, Pages 2090–2094
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us