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The importance of the effect of shear stress on endothelial cells in determining the performance of hemoglobin based oxygen carriers

Paper ID Volume ID Publish Year Pages File Format Full-Text
10816 703 2009 7 PDF Available
Title
The importance of the effect of shear stress on endothelial cells in determining the performance of hemoglobin based oxygen carriers
Abstract

The lack of blood donations and the threat of infections from blood and blood products have led to extensive research into the development of blood substitutes. The latest generation of hemoglobin based oxygen carriers (HBOC) has been shown to induce side effects like hypertension, vasoconstriction, inflammation and oxidative stress. HBOC are able to restore volemia and transport oxygen after a hemorrhagic shock, the reperfusion leading to the restoration of the blood flow in vessels. We propose an innovative approach, more closely emulating clinical situations, to assess the impact of HBOC perfusion on endothelial cells (EC) in vitro. Through this approach we quantified levels of oxidative stress, vasoactive factors and inflammation. EC were cultivated under a laminar flow to reproduce the return of shear stress (SS) during the reperfusion. We showed that heme oxygenase I transcription correlated with changes in oxidatively modified heme and methemoglobin; all were lower under SS. SS induced increased nitric oxide production, which may have implications for the mechanism of in vivo vasoconstriction and hypertension. E-selectin changes under SS were greater than those of ICAM-1. Our results demonstrate how it is essential to include SS in assays attempting to understand the potential vascular side effects of HBOC perfusion.

Keywords
Endothelial cells; Inflammation; Oxidation; Shear; Nitric oxideDex-BTC-Hb, dextran-benzene tetracarboxylate-hemoglobin; EC, endothelial cells; eNOS/iNOS, endothelial/inducible NO synthase; Hb, hemoglobin; HBOC, hemoglobin based oxygen carriers; HES, hydro
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The importance of the effect of shear stress on endothelial cells in determining the performance of hemoglobin based oxygen carriers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 4, February 2009, Pages 445–451
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us