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Cell patterning chip for controlling the stem cell microenvironment

Paper ID Volume ID Publish Year Pages File Format Full-Text
10852 704 2007 9 PDF Available
Title
Cell patterning chip for controlling the stem cell microenvironment
Abstract

Cell–cell signaling is an important component of the stem cell microenvironment, affecting both differentiation and self-renewal. However, traditional cell-culture techniques do not provide precise control over cell–cell interactions, while existing cell-patterning technologies are limited when used with proliferating or motile cells. To address these limitations, we created the Bio Flip Chip (BFC), a microfabricated polymer chip containing thousands of microwells, each sized to trap down to a single stem cell. We have demonstrated the functionality of the BFC by patterning a 50×50 grid of murine embryonic stem cells (mESCs), with patterning efficiencies >75%, onto a variety of substrates—a cell-culture dish patterned with gelatin, a 3-D substrate, and even another layer of cells. We also used the BFC to pattern small groups of cells, with and without cell–cell contact, allowing incremental and independent control of contact-mediated signaling. We present quantitative evidence that cell–cell contact plays an important role in depressing mESC colony formation, and show that E-cadherin is involved in this negative regulatory pathway. Thus, by allowing exquisite control of the cellular microenvironment, we provide a technology that enables new applications in tissue engineering and regenerative medicine.

Keywords
Cell patterning; Embryonic stem cells; Cell signaling; Microwells
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Cell patterning chip for controlling the stem cell microenvironment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 21, July 2007, Pages 3208–3216
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us