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Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
10860 705 2007 14 PDF Available
Title
Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates
Abstract

Among the parameters that can be tested in experiments on neuronal cell culture the use of different culture media and substrates represents a powerful assay to influence cell adhesion and differentiation. In this work, plasma-enhanced-chemical vapour depositions (PE-CVD) from acrylic acid and allylamine vapours have been performed to deposit coatings bearing oxygen (O)- and nitrogen (N)-containing functional groups on polyethylenetherephtalate (PET) surface. Human neuroblastoma SH-SY5Y cells were grown on plasma modified substrates and in presence of media containing different amount of fetal calf serum (FCS) or in serum-free medium containing cAMP. Our results showed that N-containing substrates improved cell adhesion, while the neurites sprouting was influenced by cell culture media. Interestingly, the presence of carboxylic groups on the modified surface can influence the expression of a differentiation marker, neurofilament-200 (NF-H), in cells grown in serum-containing media.

Keywords
Neural cell; Surface modification; Plasma polymerisation; Cell morphology
First Page Preview
Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 19, July 2007, Pages 2932–2945
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering