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The effects of cell–matrix interactions on encapsulated β-cell function within hydrogels functionalized with matrix-derived adhesive peptides

Paper ID Volume ID Publish Year Pages File Format Full-Text
10867 705 2007 8 PDF Available
Title
The effects of cell–matrix interactions on encapsulated β-cell function within hydrogels functionalized with matrix-derived adhesive peptides
Abstract

The influence of matrix-derived adhesive peptide sequences on encapsulated β-cell survival and glucose-stimulated insulin release was explored by covalently incorporating synthetic peptide sequences within a model encapsulation environment. Photopolymerized poly(ethylene glycol) (PEG) hydrogels were functionalized via the addition of acrylate-PEG-peptide conjugates to the polymer precursor solution prior to β-cell photoencapsulation. Individual MIN6 β-cells were encapsulated in the presence of the laminin-derived recognition sequences, IKLLI, IKVAV, LRE, PDSGR, RGD, and YIGSR, and the collagen type I sequence, DGEA. In the absence of cell–cell and cell–matrix contacts, encapsulated MIN6 β-cell survival diminishes within one week; however, in PEG hydrogel derivatives including the laminin sequences IKLLI and IKVAV, encapsulated β-cells exhibit preserved viability, reduced apoptosis, and increased insulin secretion. Interactions with the laminin sequences LRE, PDSGR, RGD, and YIGSR contribute to improved viability, but insulin release from these samples was not statistically greater than that from controls. MIN6 β-cells were also encapsulated with various concentrations of IKLLI and IKVAV (0.05–5.0 mm), individually, and the peptide combinations IKLLI–IKVAV, IKVAV–YIGSR, and PDSGR–YIGSR to explore synergistic effects. The presented results give evidence that synthetic peptide epitopes may be useful in the design of an islet encapsulation environment that promotes cell survival and function via targeted cell–matrix interactions.

Keywords
Cell encapsulation; Peptide; Hydrogel; β-cell; Laminin
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The effects of cell–matrix interactions on encapsulated β-cell function within hydrogels functionalized with matrix-derived adhesive peptides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 19, July 2007, Pages 3004–3011
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us