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Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness

Paper ID Volume ID Publish Year Pages File Format Full-Text
10879 706 2007 9 PDF Available
Title
Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness
Abstract

This in vitro study characterized the temporal cytokine expression profile from human monocytes exposed to phagocytosable Ti particles (0.78±0.12 μm) and to Ti discs of comparable surface roughness. Human THP-1 monocytes were cultured in six well tissue culture polystyrene (TCPS) plates. Each well was either bare, contained Ti particles (the particles were clearly engulfed by the monocytes), or contained a Ti disc. Half of the wells were treated with 1 μg/mL lipopolysaccharide (LPS), while the other half were left unstimulated. Unstimulated and LPS-stimulated cells in bare wells were the negative and positive controls, respectively. Supernatant was sampled from each well at 1, 6, 24, 48, and 72 h and assayed for the expression of nine different cytokines using a Luminex system. Three cytokines (IL-1β, GM-CSF and IL-13) gave little to no response under all conditions, while six cytokines (TNF-α, IL-6, MIP-1α, MCP-1, VEGF, and IL-1ra) were clearly detectable. Expression levels generally increased with culture time, particle concentration, and LPS stimulation. Most significantly, it was found that cells treated by Ti discs produced in many instances a higher cytokine expression than did particles.

Keywords
Ti; Cytokines; Monocytes; Endotoxin; Inflammation and wound healing
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Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 29, October 2007, Pages 4231–4239
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us