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Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

Paper ID Volume ID Publish Year Pages File Format Full-Text
10882 706 2007 11 PDF Available
Title
Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo
Abstract

Bioactive scaffolds inducing cell adhesion, differentiation have been premise for optimal formation of target tissue. Collagen has been employed as a tissue regenerative scaffold especially for bone regeneration and has been chemically surface-modified to present bioactivity. Herein, we show that peptide, denoted as collagen-binding motif (CBM, GLRSKSKKFRRPDIQYPDATDEDITSHM) identified from osteopontin (OPN) protein, was able to specifically bind collagen without chemical conjugation, while presenting apatite forming capability in vitro and in vivo. Collagen surface alone was not able to induce noticeable apatite nucleation however, mineralization was evident when assembled with CBM peptide, implying that the collagen–CBM assembly played a pivotal role in biomineralization. In vivo result further demonstrated that the CBM peptide in complex with material was able to induce bone formation by helping mineralization in the bone defect. Taken together, the CBM peptide herein and its assembly with collagen can be applied as an inducer of biomineralization as well as a bioactive scaffold for bone regeneration.

Keywords
Bioactive scaffold; Collagen-binding motif peptide; Assembly; Osteopontin; Biomineralization; Bone regenerationCBM, collagen-binding motif; SPR, surface plasmon resonance; BSP, bone sialoprotein; OPN, osteopontin; ECM, extracellular matrix; XRD, X-ray dif
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Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 29, October 2007, Pages 4257–4267
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us