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Orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction

Paper ID Volume ID Publish Year Pages File Format Full-Text
10883 706 2007 9 PDF Available
Title
Orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction
Abstract

The creation of 3D scaffolds that mimic the structure of physiological tissue required for normal cell function is a major bioengineering challenge. For corneal stroma reconstruction this necessitates the creation of a stroma-like scaffold consisting of a stack of orthogonally disposed sheets of aligned collagen fibrils. This study demonstrates that such a scaffold can be built up using magnetic alignment. By allowing neutralized acid-soluble type I collagen to gel in a horizontal magnetic field (7 T) and by combining a series of gelation–rotation–gelation cycles, a scaffold of orthogonal lamellae composed of aligned collagen fibrils has been formed. Although initially dilute, the gels can be concentrated without noticeable loss in orientation. The gels are translucent but their transparency can be greatly improved by the addition of proteoglycans to the gel-forming solution. Keratocytes align by contact guidance along the direction of collagen fibrils and respect the orthogonal design of the collagen template as they penetrate into the bulk of the 3D matrix. The scaffold is a significant step towards the creation of a corneal substitute with properties resembling those of native corneal stroma.

Keywords
Corneal stroma reconstruction; Collagen; Proteoglycans; Keratocytes; Stroma-like scaffold; Magnetic alignment
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Orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 29, October 2007, Pages 4268–4276
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us