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Effectiveness of three extraction techniques in the development of a decellularized bone–anterior cruciate ligament–bone graft

Paper ID Volume ID Publish Year Pages File Format Full-Text
10904 707 2005 11 PDF Available
Title
Effectiveness of three extraction techniques in the development of a decellularized bone–anterior cruciate ligament–bone graft
Abstract

In this study, porcine bone–anterior cruciate ligament–bone (B–ACL–B) grafts were decellularized using one of three protocols incorporating surfactants lauryl sulfate (SDS), Triton X-100, and/or an organic solvent (tributyl phosphate (TnBP)). The effectiveness of Triton–SDS, Triton–Triton or Triton–TnBP treatments in removing cellular materials was determined and possible changes in biochemical composition and mechanical properties due to each treatment were investigated. Treatment with Triton–SDS was most effective at removing cell nuclei and intracellular protein (vimentin) from the ACL but affected both the collagen and glycosaminoglycan (GAG) components of the extracellular matrix while increasing the tensile stiffness of the ligament. Triton–Triton was the least effective of the three treatments in terms of cellular extraction, but did not significantly change the mechanical and biochemical properties of the ACL. Triton–TnBP matched the level of decellularization achieved by Triton–SDS in terms of visible cell nuclei; however, the extraction of intracellular vimentin was less consistent. TnBP treatment also slightly decreased the collagen content of the ACL but did not alter its mechanical properties.Overall, all three decellularization treatments maintained adequate mechanical and biochemical properties of B–ACL–B grafts to justify the further investigation of all three decellularization protocols. The selection of a superior treatment will depend on future studies of the propensity of treated tissues for repopulation by host ACL fibroblasts and, ultimately, on any immunogenic and/or remodeling host response induced in vivo.

Keywords
Decellularized; Ligament; Anterior cruciate ligament; Allograft
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Effectiveness of three extraction techniques in the development of a decellularized bone–anterior cruciate ligament–bone graft
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 35, December 2005, Pages 7339–7349
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us