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Rapid three-dimensional biointerfaces for real-time immunoassay using hIL-18BPa as a model antigen

Paper ID Volume ID Publish Year Pages File Format Full-Text
10920 707 2005 10 PDF Available
Title
Rapid three-dimensional biointerfaces for real-time immunoassay using hIL-18BPa as a model antigen
Abstract

With the goal of designing a rapid and affordable system of real-time immune monitoring for future diagnostic applications in sepsis, we have developed a biointerface composed of polyethyleneimine (PEI) and carboxymethylcellulose (CMC) to provide a means of prompt and facile immunoassay. Biointerface assembly is complete within 30 min, with all preparation performed and monitored within the measurement chamber of a quartz crystal microgravimetry with dissipation (QCM-D) sensor. Optimised biointerface composition, as determined by the mass of antibody immobilised, the level of antigen detection, and the amount of non-specific binding of human serum albumin, was determined to consist of a 4.0 mg/mL CMC hydrogel layer cross-linked to a 0.5 mg/mL PEI sub-layer. Tapping mode atomic force microscopy (AFM) in liquid demonstrates highly uniform and smooth surfaces using these hydrogels. Sensitivity of the biointerface for rhIL-18BPa is 400 ng/mL, with detection of 1 μg/mL achievable following 25 surface regenerations. Performance of the biointerface is verified using surface plasmon resonance (SPR), demonstrating the ability of the biointerface to be applied across platforms.

Keywords
QCM; SPR; AFM; Immunoassay; Real-time; Sepsis
First Page Preview
Rapid three-dimensional biointerfaces for real-time immunoassay using hIL-18BPa as a model antigen
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 35, December 2005, Pages 7514–7523
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering