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A piezoelectric immunosensor for the detection of α-fetoprotein using an interface of gold/hydroxyapatite hybrid nanomaterial

Paper ID Volume ID Publish Year Pages File Format Full-Text
10931 708 2007 8 PDF Available
Title
A piezoelectric immunosensor for the detection of α-fetoprotein using an interface of gold/hydroxyapatite hybrid nanomaterial
Abstract

The ideal immobilization methods that are suitable for binding immuno-active materials with high efficiency onto the sensing surface are the key target to pursue in the current biosensor design. In this paper, a new hybrid material formed by assembling gold nanoparticles (GNP) onto nano-sized hydroxyapatite (HA) has been employed for the interface design of piezoelectric immunosensor, on which the antibodies were bound. The detection performances of the resulting immunosensor were investigated by use of the antibody–antigen model system of α-Fetoprotein (AFP), an important indicator in the diagnosis of clinical cancers. The hybrid material was characterized by the UV–vis spectroscopy, the SEM and TEM measurements. The frequency and electrochemical impedance responses characteristics for the processes of immobilization and immunoreaction of anchored anti-AFP antibodies were studied in detail. The immunoresponse of the proposed immunosensor was compared with those antibodies immobilized by using HA or GNP alone. It was found that the developed sensing interface has some advantages such as the activation-free immobilization and the high antigen-binding activities of antibodies. The as-prepared immunosensor can allow for the determination of AFP in the concentration range of 15.3–600.0 ng ml−1. Such an interface design with the nano-sized hybrid materials should be tailored as a new alternative used for biosensor design.

Keywords
Gold nanoparticles; Hydroxyapatite; Immunosensor; AFP
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A piezoelectric immunosensor for the detection of α-fetoprotein using an interface of gold/hydroxyapatite hybrid nanomaterial
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 12, April 2007, Pages 2147–2154
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us