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Interrelationship of micromechanics and morphology of fibroblasts adhered on different polymeric surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
1094 76 2009 9 PDF Available
Title
Interrelationship of micromechanics and morphology of fibroblasts adhered on different polymeric surfaces
Abstract

The membrane stiffness (ε) of rat lung fibroblasts (RLFs) adhered on different polymeric surfaces was probed by atomic force microscopy. The corresponding cell morphology was also analyzed to probe its interrelationship with ε. Two tyrosine-derived polymer families, poly(DTR glutarate)s and poly(DTE-co-PEG1000 carbonate)s with systematic variations in the chemical composition and physical properties, notably surface hydrophilicity, were used. The cell membrane of adhered RLFs was indented by a probe tip. ε was obtained by best-fitting the relationship of applied tip forces and the indentation depth with the Hertz model. Excluding tissue culture polystyrene, non-PEG-containing polymers are generally hydrophobic and the changes in chemical composition do not elicit significant changes in ε. In contrast, polymers containing as little as 2 mol.% PEG display a major increase in surface hydrophilicity and invoke a substantial decrease in ε. Additionally, RLFs show a high degree of spreading and fibroblastic appearance on non-PEG-containing polymers, but much less spreading and axial morphology when PEG is present. A mechanism is proposed to explain how a cell maintains its structural integrity on different polymeric surfaces: the degree of cell spreading is higher on non-PEG-containing surfaces than on PEG-containing ones, resulting in more extended cytoskeletal filaments and hence a stiffer cell membrane. Our studies shed light on the use of cellular micromechanics, and in particular membrane stiffness, to characterize cell response as a function of the chemical composition of the underlying substrata.

Keywords
Mechanical properties; Cell morphology; Atomic force microscopy; Cell spreading; Fibroblast
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Interrelationship of micromechanics and morphology of fibroblasts adhered on different polymeric surfaces
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 8, October 2009, Pages 2823–2831
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us