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The control of dendritic cell maturation by pH-sensitive polyion complex micelles

Paper ID Volume ID Publish Year Pages File Format Full-Text
10945 709 2009 9 PDF Available
Title
The control of dendritic cell maturation by pH-sensitive polyion complex micelles
Abstract

Double-hydrophilic block copolymer micelles were designed as vectors for ex vivo dendritic cell engineering to improve the delivery of therapeutic molecules in such immune cells. Polymethacrylic acid-b-polyethylene oxide (PMAA2100-b-POE5000)/poly-l-lysine micelles were optimised and showed a hydrodynamic diameter of 30 nm with a peculiar core organised with hydrogen bonds as well as hydrophobic domains. The micelles proved high stability in physiological conditions (pH and ionic strength) and were also able to disassemble under acidic conditions mimicking acidic endolysosomes. The efficient endocytosis of the optimised micelles tested on bone marrow-derived dendritic cells was monitored by fluorescence-activated cell sorting and microscopy analysis. Finally, the micelle biocompatibility permitted a complete control of the dendritic cell-maturation process widening the therapeutical potential of such engineered dendritic cells for cancer vaccines as well as for immunomodulation in autoimmune diseases.

Keywords
Polyion complex micelles; pH-sensitivity; Dendritic cell; Maturation control; Immunotherapy
First Page Preview
The control of dendritic cell maturation by pH-sensitive polyion complex micelles
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 2, January 2009, Pages 233–241
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering