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The construction of 3D-engineered tissues composed of cells and extracellular matrices by hydrogel template approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
10953 710 2007 9 PDF Available
Title
The construction of 3D-engineered tissues composed of cells and extracellular matrices by hydrogel template approach
Abstract

The three-dimensional (3D)-engineered tissues composed of only cells and extracellular matrices (ECM) were constructed by the hydrogel template approach. The disulfide-crosslinked poly(γ-glutamic acid) hydrogels were prepared as a template hydrogel. These template hydrogels were easily decomposed under physiological conditions using reductants such as cysteine, glutathione and dithiothreitol by cleavage of disulfide crosslinkage to thiol groups. The decomposed polymers are soluble in cell culture medium. The cleaving of disulfide bond was determined by UV–vis and FT-IR spectroscopies. We successfully prepared the 3D-engineered tissues (thickness/diameter, 2 mm/1 cm) composed of mouse L929 fibroblast cells and ECM by the decomposition of only the template hydrogel with cysteine after 10 days 3D-cell culture on/in the template hydrogel. The size and thickness of the 3D-engineered tissues was completely transferred from the template hydrogel. The cultured L929 cells viability in the obtained engineered tissues was confirmed by a culture test, WST-1 method and LIVE/DEAD staining assay. The engineered tissue was self-standing and highly dense composite of the cultured cells and collagen produced by the cells. This hydrogel template approach may be useful as a new class of soft-tissue engineering technology to substitute a synthetic polymer scaffold to the ECM scaffold produced from the cultured cells.

Keywords
Cell adhesion; Biodegradation; Hydrogel; Polyamino acid; Scaffold
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The construction of 3D-engineered tissues composed of cells and extracellular matrices by hydrogel template approach
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 17, June 2007, Pages 2729–2737
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us