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Colloidal gold nanoparticle modified carbon paste interface for studies of tumor cell adhesion and viability

Paper ID Volume ID Publish Year Pages File Format Full-Text
10968 711 2005 9 PDF Available
Title
Colloidal gold nanoparticle modified carbon paste interface for studies of tumor cell adhesion and viability
Abstract

A non-toxic biomimetic interface for immobilization of living cells and electrochemical exogenous effect study of cell viability was constructed by mixing colloidal gold nanoparticles in carbon paste. A new approach to study the effects of anti-tumor drug and other exogenous factors on cell viability was proposed. The nanoparticles were efficient for preserving the activity of immobilized living cells and preventing their leakage from the electrode surface. The immobilized living AsPC-1 cells (pancreatic adenocarcinoma cells derived from ascites) exhibited an irreversible voltammetric response related to the oxidation of guanine. The presence of guanine was verified by liquid chromatography–mass spectrometry. The contents of guanine in cytoplasm of each AsPC-1 and normal pancreatic cell were detected to be 370 and 22 amol, respectively. The cytotoxic effect of adriamycin resulted in a decrease in peak current of guanine. The optimal exogenous factors that affected cell viability, including pH, temperature and salt concentration of electrolyte, were just consistent with cell growth conditions in culture. This simple and rapid method could be applied for the electrochemical investigation of exogenous effect and characterization of the viability of living cells.

Keywords
Biomimetic interface; Cell immobilization; Exogenous effect; Cyclic voltammetry; AsPC-1 cells; Colloidal gold nanoparticles; Guanine; Anti-tumor drug
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Colloidal gold nanoparticle modified carbon paste interface for studies of tumor cell adhesion and viability
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 33, November 2005, Pages 6487–6495
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us