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Effects of lyophilization on the infectivity of enveloped and non-enveloped viruses in bone tissue

Paper ID Volume ID Publish Year Pages File Format Full-Text
10975 711 2005 7 PDF Available
Title
Effects of lyophilization on the infectivity of enveloped and non-enveloped viruses in bone tissue
Abstract

Recently reported qualitative experiments proved that retroviral infectivity is not destroyed by lyophilization performed on systemically infected bone and tendon. The now accomplished quantitative determination of residual infectivity for enveloped and non-enveloped viruses allows a validation of the production process regarding viral safety in freeze-dried bone transplants.The lyophilization effect on the infectivity of two non-enveloped viruses (Maus Elberfeld virus, MEV; Porcine parvovirus, PPV) and one enveloped virus (Vesicular Stomatitis virus, VSV) was examined for virus-spiked bone material in comparison to lyophilized viruses, original virus stock, and air-dried viruses. All experiments were carried out with both cell-free and cell-associated virus. Significant differences were observed regarding the reduction of virus titers (TCID50). Infectivity of VSV was reduced by about 3–4 log10 using lyophilization in presence of bone matrix and of MEV by 6–7 log10, while no substantial reduction in virus titers was observed for PPV. Lyophilization of cell-free or cell-associated virus is not sufficient to inactivate viruses completely. However, lyophilization could have an additive effect in line with other production steps used in the manufacturing process.

Keywords
EMCV, Encephalomyocarditis virus; FeLV, Feline Leukemia virus; HAV, Hepatitis A virus; HBV, Hepatitis B virus; HCV, Hepatitis C virus; HIV, Human immunodeficiency virus; MEV, Maus Elberfeld virus; moi, Multiplicity of infection; PPV, Porcine parvovirus; T
First Page Preview
Effects of lyophilization on the infectivity of enveloped and non-enveloped viruses in bone tissue
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 33, November 2005, Pages 6558–6564
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering