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Effect of isoliquiritigenin on viability and differentiated functions of human hepatocytes maintained on PEEK-WC–polyurethane membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
10982 711 2005 10 PDF Available
Title
Effect of isoliquiritigenin on viability and differentiated functions of human hepatocytes maintained on PEEK-WC–polyurethane membranes
Abstract

In this study, we tested the ability of microporous membranes synthesised from a polymeric blend of modified polyetheretherketone (PEEK-WC) and polyurethane (PU) to support long-term maintenance and differentiation of human liver cells. The effect of isoliquiritigenin (ISL), which is a component of liquorice extract, exhibiting growth stimulatory and antiproliferative dose-dependent effect was investigated by comparing cultures treated with ISL with those untreated.To this purpose, flat-sheet membranes were prepared by a blend of PEEK-WC and PU polymers by phase inverse technique. The morphological and physico-chemical properties were characterised, respectively, by scanning electron microscopy and water contact angle measurements.Human hepatocytes cultured on PEEK-WC–PU membranes were constant up to 1 month albumin production and urea synthesis as well as the synthesis of total proteins. The liver-specific functions were expressed at high levels when cells were cultured on membranes with respect to collagen. Also the biotransformation functions were maintained for all culture periods: the ISL elimination rate increased during the culture time and high values were measured up to 22 days. Thereafter, a decrease was observed. ISL stimulated the proliferation of hepatocytes cultured on both substrata but did not affect their liver-specific functions. Hepatocytes cultured on PEEK-WC–PU membranes responded very well to ISL and expressed high levels of P450 cytochrome.These results demonstrated that long-term maintenance of human liver differentiation can be achieved on PEEK-WC–PU membranes. The incubation with ISL at the investigated concentration could stimulate the proliferation of human hepatocytes in biohybrid systems.

Keywords
Membrane; Human hepatocyte; Liver-specific functions; Biotransformation; Isoliquiritigenin
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Effect of isoliquiritigenin on viability and differentiated functions of human hepatocytes maintained on PEEK-WC–polyurethane membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 33, November 2005, Pages 6625–6634
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us