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Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
10997 711 2005 8 PDF Available
Title
Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells
Abstract

The aim of this study is to evaluate a novel microspheric vector for delivery of oligonucleotides (ODN) into dendritic cells (DC). A requirement of decoy-based modulation of transcriptional activities in DC is that the ODN would have to accumulate inside the cell. Using an ex vivo DC culture model, we demonstrate that anionic microspheres (MS) coated with an ornithine/histadine-based cationic peptide (O10H6) is an effective carrier of short ODN. This method does not disrupt the colloidal nature of the microspheric particles. The MS provide stabilizing effect on DNA and O10H6 complexation. Accumulation of ODN in DC is greatly enhanced with the surface modified MS. Taken together, these data demonstrate that the self assembly system of MSO10H6 is an effective delivery vehicle for DNA-based modulation of DC functions.

Keywords
ODN, oligonucleotides; MS, microspheres; O10H6, a peptide consists of (from the N-terminal) 10 ornithine residues followed by six histidines; DC, dendritic cells.DNA delivery; Dendritic cell; Nanoparticle; Cationic peptide; Oligonucleotide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 33, November 2005, Pages 6754–6761
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us