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Tuning the sol–gel microenvironment for acetylcholinesterase encapsulation

Paper ID Volume ID Publish Year Pages File Format Full-Text
10999 711 2005 9 PDF Available
Title
Tuning the sol–gel microenvironment for acetylcholinesterase encapsulation
Abstract

The effect of the sol–gel microenvironment on the activity of acetylcholinesterase, an enzyme of high bio-analytical interest, is presented and is correlated to the overall analytical performance of corresponding biosensors. The sol–gel membranes are initially optimized with respect to the catalyst and the TEOS:H2O ratio (r), for mechanical stability, porosity, and hydrophobicity as well as in terms of enzymatic activity. FT-IR and electrochemical impedance spectroscopy (EIS) are used to probe the configuration and rotational mobility of the enzyme within the sol–gel matrices. Overall, it is observed that the rotational mobility of the protein can be correlated with the sensitivity of the biosensor. Optimum biosensor performance is obtained for base-catalyzed sol–gels with r values close to 2. The biosensor has sensitivity of 2.5 μA/mm, a linear range of response between 1 and 3 mm, response time of about 30 s, and sensor-to-sensor reproducibility (RSD) of 3%. These analytical characteristics are far superior to previously reported sol–gel biosensors.

Keywords
Acetylcholinesterase; Sol–gel; Infrared spectroscopy; Electrochemical impedance spectroscopy; Amperometric biosensor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 33, November 2005, Pages 6771–6779
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us