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Antibacterial nitric oxide-releasing xerogels: Cell viability and parallel plate flow cell adhesion studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
11006 712 1956 9 PDF Available
Title
Antibacterial nitric oxide-releasing xerogels: Cell viability and parallel plate flow cell adhesion studies
Abstract

The ability of nitric oxide (NO)-releasing xerogels to reduce adhesion of Pseudomonas aeruginosa under flowing conditions was evaluated using a parallel plate flow chamber. At a controlled bacterial suspension flow rate of 0.2 mL/min, the NO-releasing xerogels reduced bacterial adhesion in a flux-dependent fashion, with an NO flux of ∼21 pmol cm−2 s−1 reducing P. aeruginosa adhesion by ∼65% compared to controls. Fluorescent viability staining indicated that bacteria adhered to NO-releasing xerogels were killed within 7 h. Quantitative cell-plating viability studies showed that the extent of bactericidal activity was dependent on the total amount of NO released, with 750 nmol cm−2 killing >90% more adhered bacteria than xerogels releasing 25 nmol cm−2. Thus, NO-releasing xerogels were shown to both inhibit P. aeruginosa adhesion and kill adhered bacteria cells, two important steps toward designing anti-infective biomaterial coatings.

Keywords
Nitric oxide; Xerogel; Bacterial adhesion; Cell viability; Implant infection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 11, April 2007, Pages 1948–1956
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us