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Nanoparticles of poly(lactide)—tocopheryl polyethylene glycol succinate (PLA-TPGS) copolymers for protein drug delivery

Paper ID Volume ID Publish Year Pages File Format Full-Text
11015 712 2007 10 PDF Available
Title
Nanoparticles of poly(lactide)—tocopheryl polyethylene glycol succinate (PLA-TPGS) copolymers for protein drug delivery
Abstract

Nanoparticles (NPs) of poly(lactide)-tocopheryl polyethylene glycol succinate (PLA-TPGS) copolymers with various PLA:TPGS component ratios were prepared by the double emulsion technique for protein drug formulation with bovine serum albumin (BSA) as a model protein. Influence of the PLA:TPGS component ratio and the BSA loading level on the drug encapsulation efficiency (EE) and in vitro drug release behavior was investigated. The PLA-TPGS NPs achieved 16.7% protein drug loading and 75.6% EE, which exhibited a biphasic pattern of controlled protein release with higher initial burst for those NPs of more TPGS content. Furthermore, the released proteins retained good structural integrity for at least 35 days at 37 °C as indicated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism (CD) spectroscopy. Compared with other biodegradable polymeric NPs such as poly(d,l-lactide-co-glycolide) (PLGA) NPs, PLA-TPGS NPs could provide the encapsulated proteins a milder environment. Confocal laser scanning microscopy (CLSM) observation demonstrated the intracellular uptake of the PLA-TPGS NPs by NIH-3T3 fibroblast cells and Caco-2 cancer cells. This research suggests that PLA-TPGS NPs could be of great potential for clinical formulation of proteins and peptides.

Keywords
Biodegradable polymers; Cancer nanotechnology; Bovine serum albumin; Nanomedicine; Protein drug delivery
First Page Preview
Nanoparticles of poly(lactide)—tocopheryl polyethylene glycol succinate (PLA-TPGS) copolymers for protein drug delivery
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 11, April 2007, Pages 2041–2050
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering