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The effect of actin disrupting agents on contact guidance of human embryonic stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
11025 713 2007 10 PDF Available
Title
The effect of actin disrupting agents on contact guidance of human embryonic stem cells
Abstract

Mammalian cells respond to their substrates by complex changes in gene expression profiles, morphology, proliferation and migration. We report that substrate nanotopography alters morpohology and proliferation of human embryonic stem cells (hESCs). Fibronectin-coated poly(di-methyl siloxane) substrates with line-grating (600 nm ridges with 600 nm spacing and 600±150 nm feature height) induced hESC alignment and elongation, mediated the organization of cytoskeletal components including actin, vimentin, and α-tubulin, and reduced proliferation. Spatial polarization of gamma-tubulin complexes was also observed in response to nanotopography. Furthermore, the addition of actin disrupting agents attenuated the alignment and proliferative effects of nanotopography. These findings further demonstrate the importance of interplay between cytoskeleton and substrate interactions as a key modulator of morphological and proliferative cellular response in hESCs on nanotopography.

Keywords
Stem cell; Microstructure; Cell morphology; Cell proliferation
First Page Preview
The effect of actin disrupting agents on contact guidance of human embryonic stem cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 28, October 2007, Pages 4068–4077
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering