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Towards control of smooth muscle cell differentiation in synthetic 3D scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
11044 714 2008 10 PDF Available
Title
Towards control of smooth muscle cell differentiation in synthetic 3D scaffolds
Abstract

A central tenant of tissue engineering is that cells should be able to recapitulate full functional tissue capability when placed within an appropriate architecture or scaffold. The aim of this study was to examine the effect of three-dimensional (3D) architecture on the differentiated phenotype of human smooth muscle cells derived from the stroma of the lower urinary tract. Stromal cell cultures were established from surgical specimens and the differentiated smooth muscle cell phenotype was monitored by gene expression, immunofluorescence and immunoblotting. Expression of contractile proteins, including smooth muscle myosin and smoothelin, was lost by cultures grown on two-dimensional (2D) tissue culture polystyrene, but was regained to some extent by the removal of serum and by the addition of TGFβ1. Stromal cells were seeded onto plasma-coated electrospun polystyrene scaffolds to examine the influence of 3D architecture on smooth muscle cell phenotype, but differentiation was inhibited by serum proteins that adsorbed non-specifically onto the large surface area of the scaffold. Stromal cells failed to adhere to the scaffold in serum-free conditions, but laminin pre-coating of the scaffold prevented serum adsorption and promoted cell attachment and differentiation. The study highlights how non-specific factors, such as serum adsorption, may confound the development of materials for tissue engineering.

Keywords
Bladder; Tissue culture; Smooth muscle cell; Differentiation; Electrospinning; Polystyrene; Scaffold
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Towards control of smooth muscle cell differentiation in synthetic 3D scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 23, August 2008, Pages 3357–3366
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us