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The effect of cholecyst-derived extracellular matrix on the phenotypic behaviour of valvular endothelial and valvular interstitial cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
11050 715 2007 9 PDF Available
Title
The effect of cholecyst-derived extracellular matrix on the phenotypic behaviour of valvular endothelial and valvular interstitial cells
Abstract

Cholecyst-derived extracellular matrix (CEM) is a novel, proteinaceous biomaterial, derived from the porcine cholecyst, which may have potential applications as a scaffold in the area of heart valve tissue engineering. In this study the potential of CEM to support the proliferation of valvular endothelial cells (VECs) and valvular interstitial cells (VICs), while maintaining their phenotypic mRNA synthesis, protein expression and morphology was assessed by biochemical assays, electron microscopy, immunostaining and reverse-transcriptase polymerase chain reaction. VICs and VECs were isolated from the porcine aortic valve and techniques were developed for the isolation of CEM for cell culture. VECs and VICs cultured on CEM adhered and proliferated, maintaining their phenotypic morphology. VECs synthesised von Willebrand factor mRNA and endothelial nitric oxide synthase (eNOS) mRNA and expressed eNOS and VICs synthesised alpha-smooth muscle actin (αSMA) mRNA and expressed αSMA. Cellular area fraction of VICs expressing αSMA was 87.7±6.8% and cellular area fraction of VECs expressing eNOS was 93.8±9.3%. Findings of this study support the hypothesis that CEM is a potential biomaterial for tissue engineered heart valve scaffold design.

Keywords
Cardiac tissue engineering; Heart valve; Biomimetic material; Endothelialisation; Scaffold
First Page Preview
The effect of cholecyst-derived extracellular matrix on the phenotypic behaviour of valvular endothelial and valvular interstitial cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 8, March 2007, Pages 1461–1469
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering