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Influence of glass and polymer coatings on CHO cell morphology and adhesion

Paper ID Volume ID Publish Year Pages File Format Full-Text
11062 715 2007 13 PDF Available
Title
Influence of glass and polymer coatings on CHO cell morphology and adhesion
Abstract

Successful development of cell-on-chip microsystems where living cells are deposited and grown in microfabricated structures is highly dependent on the control of cell/substrate interactions. In this study, several materials of interest were tested for CHO cell growth and morphology: (i) glass, fibronectin-, poly-l-lysine- and 3-aminopropyltriethoxysilane (APTES)—treated glass and UV/O3-modified PDMS coating on glass as well as (ii) silicon, poly-l-lysine-, APTES-, O2 plasma-treated and oxide-coated silicon. In addition, we quantitatively characterized cell adhesion to these substrates using a radial flow detachment assay. Lack of correlation between cell adhesion and cell morphology was systematically observed for all substrates. In particular, we show that PDMS coatings on glass can be finely tuned by UV/O3 treatment to enhance cell adhesion and induce elongated morphology. Moreover, we observed a low shear stress cell detachment mechanism on silicon oxide coatings on silicon wafers. It is therefore possible with these coatings to selectively influence either cell adhesion or morphology.

Keywords
Cell adhesion; Cell morphology; Surface energy; Surface coatings; Biochips
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 8, March 2007, Pages 1572–1584
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us