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Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
11074 716 2005 10 PDF Available
Title
Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold
Abstract

Mesenchymal stromal cells (MSCs) seem to be a good alternative to chondrocytes for cartilage regeneration. To obtain new information on the sequence of cellular and molecular events during in vitro chondrogenic differentiation we analysed MSCs on a widely used hyaluronic acid biomaterial (Hyaff®-11). Cellular differentiation was induced using two different concentrations of TGFβ1TGFβ1 (10 and 20 ng/ml) and the process was analysed at different time points (24 h, and 7, 14, 21 and 28 days) using techniques of light and electron microscopy, real-time PCR and immunohistochemistry. We found that without TGFβTGFβ MSCs did not survive while in the presence of TGFβTGFβ the cells significantly proliferated from day 7 until day 28. Light and electron microscopy showed that TGFβTGFβ at 20 ng/ml better induced the formation of cartilage-like tissue. Real-time PCR showed an increased expression of collagen type II, IX and aggrecan associated to a down-regulation of collagen type I. Immunohistochemical analysis confirmed that collagen type I was down-modulated while collagen type II increased from day 14 to day 28.These data clearly showed that higher concentrations of TGFβTGFβ (20 ng/ml) induce chondrogenesis of MSCs on Hyaff®-11 scaffold better than 10 ng/ml of TGFβTGFβ. This process is characterized by a sequence of cellular and molecular events that deal with the in vitro formation of a cartilage-like tissue.

Keywords
Mesenchymal stromal cells; Chondrogenesis; Biomaterial; Tissue engineering
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Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 28, October 2005, Pages 5677–5686
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us