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Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
11080 716 2005 9 PDF Available
Title
Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins
Abstract

Two kinds of molecularly imprinted polymers were prepared using hemoglobin as the imprinting molecule, acrylamide as the functional monomer, chitosan beads and maleic anhydride-modified chitosan beads as matrixes, respectively. Static adsorbing experimental results showed that an equal class of adsorption was formed in the imprinted polymers and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated. Chromatographic characteristics showed that the column bedded with the hemoglobin imprinted beads could separate hemoglobin and bovine serum albumin effectively from their mixture, which indicates that the imprinted beads have very higher selectivity for hemoglobin than the non-imprinted with the same chemical composition.

Keywords
Chitosan beads; Polyacrylamide; Protein separation; Molecular imprinting
First Page Preview
Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 28, October 2005, Pages 5737–5745
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering