fulltext.study @t Gmail

Endogenous overexpression of hyaluronan synthases within dynamically cultured collagen gels: Implications for vascular and valvular disease

Paper ID Volume ID Publish Year Pages File Format Full-Text
11087 717 2008 8 PDF Available
Title
Endogenous overexpression of hyaluronan synthases within dynamically cultured collagen gels: Implications for vascular and valvular disease
Abstract

Hyaluronan is a ubiquitous component of the extracellular matrix with important roles in cell and tissue functions. Hyaluronan content is often elevated in cardiovascular diseases, such as mitral valve disease and atherosclerosis. The objective of this study was to determine the impact of endogenously produced hyaluronan dynamically cultured three-dimensional model of collagenous tissues. Collagen gels containing excess HA and hyaluronan synthase (has) overexpressing cells were grown in a cyclic strain environment to simulate cell-mediated matrix organization. Cyclic strain caused a significant elevation in the collagen fibril density, cell number, and hyaluronan content of the resulting collagen gels compared to those grown under a static strain regimen. The material behavior of collagen gels containing has overexpressing cells was also notably weakened compared to controls. Transmission electron microscopy and immunohistochemistry showed that proteoglycan distribution was influenced by both strain and has overexpression. The results were also dependent on the specific has isozyme overexpressed. This investigation helps to identify the mechanism by which hyaluronan acts in vivo to alter tissue material behavior in cardiovascular diseases such as myxomatous mitral valve disease and atherosclerosis.

Keywords
Hyaluronic acid/hyaluronan; ECM (extracellular matrix); Collagen; Mechanical properties; Smooth muscle cells
First Page Preview
Endogenous overexpression of hyaluronan synthases within dynamically cultured collagen gels: Implications for vascular and valvular disease
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 20, July 2008, Pages 2969–2976
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us