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In vitro and in vivo characteristics of PCL scaffolds with pore size gradient fabricated by a centrifugation method

Paper ID Volume ID Publish Year Pages File Format Full-Text
11116 719 2007 8 PDF Available
Title
In vitro and in vivo characteristics of PCL scaffolds with pore size gradient fabricated by a centrifugation method
Abstract

Polycaprolactone (PCL) cylindrical scaffolds with gradually increasing pore size along the longitudinal direction were fabricated by a novel centrifugation method to investigate pore size effect on cell and tissue interactions. The scaffold was fabricated by the centrifugation of a cylindrical mold containing fibril-like PCL and the following fibril bonding by heat treatment. The scaffold showed gradually increasing pore size (from ∼88 to ∼405 μm) and porosity (from ∼80% to ∼94%) along the cylindrical axis by applying the centrifugal speed, 3000 rpm. The scaffold sections were examined for their in vitro cell interactions using different kinds of cells (chondrocytes, osteoblasts, and fibroblasts) and in vivo tissue interactions using a rabbit model (skull bone defects) in terms of scaffold pore sizes. It was observed that different kinds of cells and bone tissue were shown to have different pore size ranges in the scaffold for effective cell growth and tissue regeneration. The scaffold section with 380–405 μm pore size showed better cell growth for chondrocytes and osteoblasts, while the scaffold section with 186–200 μm pore size was better for fibroblasts growth. Also the scaffold section with 290–310 μm pore size showed faster new bone formation than those of other pore sizes. The pore size gradient scaffolds fabricated by the centrifugation method can be a good tool for the systematic studies of the interactions between cells or tissues and scaffolds with different pore size.

Keywords
Pore size; Scaffold; Porosity; Polycaprolactone (PCL); Cell adhesion; Bone regeneration
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In vitro and in vivo characteristics of PCL scaffolds with pore size gradient fabricated by a centrifugation method
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 9, March 2007, Pages 1664–1671
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us