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Morphological regulation of rabbit chondrocytes on glucose-displayed surface

Paper ID Volume ID Publish Year Pages File Format Full-Text
11118 719 2007 9 PDF Available
Title
Morphological regulation of rabbit chondrocytes on glucose-displayed surface
Abstract

A culture surface was designed to regulate morphology of rabbit chondrocytes by changing the ratio of d- and l-glucose isomers displayed on a glass plate. With increasing ratio of d-glucose displayed on the surfaces, the efficiency of cell attachment improved, meaning that the attachment exclusively occurred via mediation of an affinity between d-glucose displayed and glucose transporter on cell membrane. At 0% and 100% d-glucose display, the round-shaped cells appeared dominantly, and most of cells became stretched in shape at 50% d-glucose display, indicating that the frequency of round-shaped cells depicted a concave profile against the ratio of d-glucose displayed. From the cytoskeletal staining of F-actin and vinculin, the immature stress fibers with fewer focal contacts were recognized in both the round shaped cells and those stretched in shape on 100% d-glucose-displayed surface. The time-lapse observation revealed that the cells on 100% d-glucose-displayed surface conducted active migration and aggregation with formation of collagen type II. These results suggest that 100% d-glucose-displayed surface can offer culture environment to maintain the chondrocytic phenotype of cells, similarly to the conditions achieved in three-dimensional (3-D) culture.

Keywords
Chondrocyte; Morphology; Glucose-displayed surface; ECM formation; Cytoskeletal formation
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Morphological regulation of rabbit chondrocytes on glucose-displayed surface
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 9, March 2007, Pages 1680–1688
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us