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Novel silk sericin/gelatin 3-D scaffolds and 2-D films: Fabrication and characterization for potential tissue engineering applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
1112 76 2009 14 PDF Available
Title
Novel silk sericin/gelatin 3-D scaffolds and 2-D films: Fabrication and characterization for potential tissue engineering applications
Abstract

In this study, we report for the first time the fabrication of novel 3-D sericin/gelatin scaffolds and 2-D films using non-mulberry Antheraea mylitta silk cocoon sericin protein. The matrices were fabricated, biophysically characterized and optimized for cell culture applications. Blended sericin/gelatin 3-D scaffolds were highly porous with an optimum pore size of 170 ± 20 μm. The scaffolds were robust with enhanced mechanical strength and showed high compressibility. Swelling studies showed high swellability along with complete degradation in the presence of phosphate-buffered saline. Cytocompatibility of the matrices was evaluated using feline fibroblasts showing normal spreading and proliferation as assessed by fluorescence microscopy. Cell cycle analysis showed cytocompatibility without any cell cycle arrests. Low immunogenicity of the matrices as observed through tumor necrosis factor α release reveal its potential as future biopolymeric graft material. The results of this novel study lay the foundation for the use of the silk cocoon protein sericin as a biocompatible biopolymer for tissue engineering applications.

Keywords
Silk sericin protein; Scaffolds; Biopolymers; Biocompatibility; Tissue engineering
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Novel silk sericin/gelatin 3-D scaffolds and 2-D films: Fabrication and characterization for potential tissue engineering applications
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 8, October 2009, Pages 3007–3020
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us