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Encapsulation of α-amylase into starch-based biomaterials: An enzymatic approach to tailor their degradation rate

Paper ID Volume ID Publish Year Pages File Format Full-Text
1113 76 2009 10 PDF Available
Title
Encapsulation of α-amylase into starch-based biomaterials: An enzymatic approach to tailor their degradation rate
Abstract

This paper reports the effect of α-amylase encapsulation on the degradation rate of a starch-based biomaterial. The encapsulation method consisted in mixing a thermostable α-amylase with a blend of corn starch and polycaprolactone (SPCL), which were processed by compression moulding to produce circular disks. The presence of water was avoided to keep the water activity low and consequently to minimize the enzyme activity during the encapsulation process. No degradation of the starch matrix occurred during processing and storage (the encapsulated enzyme remained inactive due to the absence of water), since no significant amount of reducing sugars was detected in solution. After the encapsulation process, the released enzyme activity from the SPCL disks after 28 days was found to be 40% comparatively to the free enzyme (unprocessed). Degradation studies on SPCL disks, with α-amylase encapsulated or free in solution, showed no significant differences on the degradation behaviour between both conditions. This indicates that α-amylase enzyme was successfully encapsulated with almost full retention of its enzymatic activity and the encapsulation of α-amylase clearly accelerates the degradation rate of the SPCL disks, when compared with the enzyme-free disks. The results obtained in this work show that degradation kinetics of the starch polymer can be controlled by the amount of encapsulated α-amylase into the matrix.

Keywords
Starch-based biomaterials; Controlled degradation rate; Thermostable α-amylase; Encapsulation; Enzyme activity
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Encapsulation of α-amylase into starch-based biomaterials: An enzymatic approach to tailor their degradation rate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 8, October 2009, Pages 3021–3030
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us