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Disassembly of layer-by-layer films of plasmid DNA and reducible TAT polypeptide

Paper ID Volume ID Publish Year Pages File Format Full-Text
11136 720 2007 8 PDF Available
Title
Disassembly of layer-by-layer films of plasmid DNA and reducible TAT polypeptide
Abstract

This paper reports the disassembly of layer-by-layer (LbL) films of plasmid DNA and a reducible cationic polypeptide. To utilize a reducing microenvironment of cellular plasma membrane as a potential trigger, LbL films are assembled to contain both DNA and the TAT-based polypeptide (PTAT) with reducible disulfide bonds in the backbone. The assembly and disassembly processes are monitored by goniometry, ellipsometry, and atomic force microscopy (AFM). The structure of the PTAT films is compared with that of non-reducible poly(l-lysine) (PLL) films. Both PTAT and PLL films exhibit exponential growth but with the contact angle alternating between characteristic values. Ellipsometry and AFM show a gradual and complete disassembly of the PTAT but not the PLL films in a 24 h period in the reducing environment in vitro. This study suggests a potential of using reducible LbL films for controlled DNA delivery.

Keywords
Layer-by-layer; Polyelectrolytes; DNA; TAT; PLL; Gene delivery
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Disassembly of layer-by-layer films of plasmid DNA and reducible TAT polypeptide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 1, January 2007, Pages 117–124
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us