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The effect of surface chemistry on the formation of thin films of native fibrillar collagen

Paper ID Volume ID Publish Year Pages File Format Full-Text
11139 721 2007 10 PDF Available
Title
The effect of surface chemistry on the formation of thin films of native fibrillar collagen
Abstract

In this study, we used well-defined, homogeneous, gradient and patterned substrates to explore the effects of surface chemistry on the supramolecular structure of adsorbed type I collagen. Type I collagen (320 μg/mL) was allowed to adsorb onto self-assembled CH3-, COOH-, NH2- and OH-terminated alkylthiolate monolayers at 37  °C. Atomic force microscopy, ellipsometry and phase microscopy indicated that large supramolecular collagen fibril structures (approximately 200 nm in diameter, several microns long) assembled only at the hydrophobic CH3-terminated surfaces. By varying the surface energy using a mixture of OH- and CH3-terminated thiols during monolayer formation, we found that large fibril assembly occurred at surfaces with a water contact angle above 83°, but not on surfaces with a water contact angle below 63°. Examining a surface with a linear hydrophobicity gradient revealed that the assembly of large collagen fibrils requires a hydrophobic surface with a water contact angle of at least 78°. Collagen fibril density increased over a narrow range of surface energy and reached near-maximum density on surfaces with a water contact angle of 87°. These studies confirm that the supramolecular structure of adsorbed collagen is highly dependent on the underlying substrate surface chemistry. We can take advantage of this dependency to pattern areas of fibrillar and non-fibrillar collagen on a single surface. Morphology studies with vascular smooth muscle cells indicated that only collagen films formed on hydrophobic substrates mimicked the biological properties of fibrillar collagen gels.

Keywords
Collagen structure; Protein adsorption; Surface modification; AFM; Micropatterning; Smooth muscle cells
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The effect of surface chemistry on the formation of thin films of native fibrillar collagen
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 4, February 2007, Pages 576–585
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us