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Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
11181 723 2008 12 PDF Available
Title
Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds
Abstract

Autologous bone grafts are currently the gold standard for treatment of large bone defects, but their availability is limited due to donor site morbidity. Different substitutes have been suggested to replace these grafts, and this study presents a bone tissue engineered alternative using silicate-substituted tricalcium phosphate (Si-TCP) scaffolds seeded with human bone marrow-derived mesenchymal stem cells (hMSC). The cells were seeded onto the scaffolds and cultured either statically or in a perfusion bioreactor for up to 21 days and assessed for osteogenic differentiation by alkaline phosphatase activity assays and by quantitative real-time RT-PCR on bone markers. During culture, cells from the flow cultured constructs demonstrated improved proliferation and osteogenic differentiation verified by a more pronounced expression of several bone markers, e.g. alkaline phosphatase, osteopontin, Runx2, bone sialoprotein II, and bone morphogenetic protein 2. Cells and matrix were distributed homogenously throughout the entire scaffold in flow culture, whereas only a peripheral layer was obtained after static culture. A viable and homogenous ex vivo bone construct with superior osteogenic properties was produced in dynamic culture and may provide a replacement for autologous grafts.

Keywords
Bioreactor; Stem cells; Silicate-substituted tricalcium phosphate; Scaffold; Osteogenesis; Real-time RT-PCRALP, alkaline phosphatase; BMP2, bone morphogenetic protein 2; BSPII, bone sialoprotein II; coll I, collagen I; EthD-1, ethidium homodimer; HA, hydr
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Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 17, June 2008, Pages 2616–2627
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us