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Targeted delivery of paclitaxel using folate-decorated poly(lactide)–vitamin E TPGS nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
11186 723 2008 10 PDF Available
Title
Targeted delivery of paclitaxel using folate-decorated poly(lactide)–vitamin E TPGS nanoparticles
Abstract

We synthesized nanoparticles (NPs) of the blend of two-component copolymers for targeted chemotherapy with paclitaxel used as model drug. One component is poly(lactide)–d-α-tocopheryl polyethylene glycol succinate (PLA–TPGS), which is of desired hydrophobic–lipophilic balance, and another is TPGS–COOH, which facilitates the folate conjugation for targeting. The nanoparticles of the two-copolymer blend at various component ratio were prepared by the solvent extraction/evaporation single emulsion method and then decorated by folate, which were characterized by laser light scattering (LLS) for particles' size and size distribution, zeta potential analyzer for surface charge, and X-ray photoelectron spectroscopy (XPS) for surface chemistry. The drug encapsulation efficiency (EE) and in vitro drug release were measured by high performance liquid chromatography (HPLC). The targeting effect was investigated in vitro by cancer cell uptake of coumarin-6-loaded NPs and further confirmed by cytotoxicity of cancer cells treated with the drug formulated in the NPs. We showed that the NP formulation has great advantages vs the pristine drug in achieving better therapeutic effect, which increased 8.68% for MCF-7 breast cancer cells, and that the folate-decoration can significantly promote targeted delivery of the drug into the corresponding cancer cells and thus enhance its therapeutic effect, which increased 24.4% for the NP formulation of 16.7% TPGS–COOH component and 31.1% for the NP formulation of 33.3% TPGS–COOH component after 24 h treatment at the same 25 μg/ml paclitaxel concentration. The experiments on C6 glioma cells further confirmed these advantages.

Keywords
Cancer nanotechnology; Biodegradable polymers; Breast cancer; Nanobiotechnology; Nanomedicine
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Targeted delivery of paclitaxel using folate-decorated poly(lactide)–vitamin E TPGS nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 17, June 2008, Pages 2663–2672
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us