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Reversible electronic nanoswitch based on DNA G-quadruplex conformation: A platform for single-step, reagentless potassium detection

Paper ID Volume ID Publish Year Pages File Format Full-Text
11189 723 2008 8 PDF Available
Title
Reversible electronic nanoswitch based on DNA G-quadruplex conformation: A platform for single-step, reagentless potassium detection
Abstract

A novel on/off electronic nanoswitch is for the first time described based on the conformational change of DNA sequence possessing a single guanine (G)-rich stretch. Here, a thiolated, amine-containing G-rich DNA sequence is immobilized on the surface of gold electrode by means of facile sulfur–gold chemistry, followed by being labeled with redox-active ferrocene molecules serving as the signaling species. The surface-confined DNA sequence is able to change its configuration between rigid tetramolecular G-quadruplex and flexible single-stranded structures. The large conformational change enables the probes to perform an inchworm like extending–shrinking motion, which is reflected by the fluctuation in current intensity that depends on the electron-transfer distance between the electrode surface and the redox labels. Since potassium ion can specifically bind to G-quadruplex, using this reagentless reusable electrochemical sensing platform, the simple, rapid and selective detection of potassium ion can be accomplished without the use of exogenous reagents. Success in the present electronic nanoswitch is expected to promote the exploitation of functional DNA-based nanosystems.

Keywords
G-quadruplex structure; Conformational change; Electronic nanoswitch
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Reversible electronic nanoswitch based on DNA G-quadruplex conformation: A platform for single-step, reagentless potassium detection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 17, June 2008, Pages 2689–2696
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us