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The effect of purmorphamine on osteoblast phenotype expression of human bone marrow mesenchymal cells cultured on titanium

Paper ID Volume ID Publish Year Pages File Format Full-Text
11196 724 2005 4 PDF Available
Title
The effect of purmorphamine on osteoblast phenotype expression of human bone marrow mesenchymal cells cultured on titanium
Abstract

Purmorphamine is a new molecule with osteogenesis-inducing activity in multipotent progenitor cells. The aim of this study was to evaluate whether purmorphamine maintains its osteogenic potential on human bone marrow mesenchymal cells cultured on commercially pure titanium (cpTi). Cells were cultured either in the absence or presence of purmorphamine 3 μm on cpTi in supplemented α-MEM. At 7, 14, and 21 days, cell proliferation, viability, total protein content, collagen content, and alkaline phosphatase (ALP) activity were evaluated. Bone-like nodule formation was evaluated at 21 days. All experiments were done in quintuplicate and data were compared by ANOVA or t-test. Purmorphamine did not affect cell proliferation (p=0.619), viability (p=0.831), and collagen content (p=0.088). Total protein content (p=0.047), ALP activity (p=0.001), and bone-like nodule formation (p=0.002) were increased by purmorphamine. The present results indicate that events related to osteoblast differentiation, including increased ALP activity and bone-like nodule formation, are enhanced by purmorphamine in the presence of cpTi. It means that this molecule could be useful as an adjunct therapy to improve the osseointegration of the implants in the fields of dentistry and orthopaedics.

Keywords
Cell differentiation; Human bone marrow mesenchymal cells; Osteoblast; Purmorphamine; Titanium
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The effect of purmorphamine on osteoblast phenotype expression of human bone marrow mesenchymal cells cultured on titanium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 20, July 2005, Pages 4245–4248
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us