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The effect of gelatin–chondroitin sulfate–hyaluronic acid skin substitute on wound healing in SCID mice

Paper ID Volume ID Publish Year Pages File Format Full-Text
11218 725 2006 9 PDF Available
Title
The effect of gelatin–chondroitin sulfate–hyaluronic acid skin substitute on wound healing in SCID mice
Abstract

Tissue-engineered skin substitutes provided a feasibility to overcome the shortage of skin autograft by culturing keratinocytes and dermal fibroblasts in vitro. In this study, we applied bi-layer gelatin–chondrointin-6-sulfate–hyaluronic acid (gelatin–C6S–HA) biomatrices onto the severe combined immunodeficiency (SCID) mice to evaluate its effect on promoting wound healing. Human foreskin keratinocytes and dermal fibroblasts were cultured with reconstructed skin equivalent (rSE) for 7 days. The rSE was then grafted to the dorsum of SCID mice to evaluate its biocompatibility by histologic and immunohistochemistry analysis. The results showed that human epidermis were well-developed with the expression of differentiated markers and basement membrane-specific proteins at 4 weeks. After implantation, the percentages of skin graft take were satisfactory, while cell-seeded group was better than non-cell-seeded one. The basement membrane proteins including laminin, type IV collagen, type VII collagen, integrin α6, and integrin β4 were all detected at the dermal–epidermal junction, which showed a continuous structure in the 4 weeks after grafting. This bi-layer gelatin–C6S–HA skin substitute not only has positive effect on promoting wound healing, but also has high rate of graft take. This rSE would have the potential to be applied on the extensively and deeply burned patients who suffer from severe skin defect in the near future.

Keywords
Wound healing; Transplantation; Biomimetic material
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The effect of gelatin–chondroitin sulfate–hyaluronic acid skin substitute on wound healing in SCID mice
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 33, November 2006, Pages 5689–5697
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us