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Fabrication of hydroxyapatite ultra-thin layer on gold surface and its application for quartz crystal microbalance technique

Paper ID Volume ID Publish Year Pages File Format Full-Text
11225 725 2006 7 PDF Available
Title
Fabrication of hydroxyapatite ultra-thin layer on gold surface and its application for quartz crystal microbalance technique
Abstract

We present a method for coating gold quartz crystal microbalance with dissipation (QCM-D) sensor with ultra-thin layer of hydroxyapatite nanocrystals evenly covering and tightly bound to the surface. The hydroxyapatite layer shows a plate-like morphology and less than 20 nm in thickness. The hydroxyapatite sensor operated in liquid with high stability and sensitivity. The in-situ adsorption mechanism and conformational change of fibrinogen on gold, titanium and hydroxyapatite surfaces were investigated by QCM-D technique and Fourier-transform infrared spectroscopy. The change of secondary structures of fibrinogen adsorbed on the surfaces depended on the adsorbed amounts of protein. The secondary structure of fibrinogen adsorbed on the surfaces changes with increasing coverage. This is explained by repulsion among fibrinogens, affecting water structure and thus the strength of fibrinogen interactions on the surface. The study indicates that the hydroxyapatite sensor is applicable for qualitative and conformational analysis of protein adsorption.

Keywords
Hydroxyapatite; QCM-D; FT-IR; Fibrinogen; Adsorption
First Page Preview
Fabrication of hydroxyapatite ultra-thin layer on gold surface and its application for quartz crystal microbalance technique
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 33, November 2006, Pages 5748–5754
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering