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Activation of phospholipase D1 by surface roughness of titanium in MG63 osteoblast-like cell

Paper ID Volume ID Publish Year Pages File Format Full-Text
11231 726 2006 10 PDF Available
Title
Activation of phospholipase D1 by surface roughness of titanium in MG63 osteoblast-like cell
Abstract

Although it is recognized that the surface roughness of titanium (Ti) promotes the osteogenic differentiation, the related mechanisms and factors remain elusive. The purpose of this study was to explore the potential correlation among phospholipase D (PLD) activity, Ti surface roughness and subsequent osteoblast differentiation. The machined Ti disks were sandblasted with aluminum oxide particles to produce surfaces of varying roughness (n=160n=160). Normal or transfected MG63cells with PLD genes were cultured on roughened Ti specimens and assayed for PLD, alkaline phosphatase (ALP) and osteocalcin. The statistical significance was evaluated by analysis of variance. The activity, mRNA and protein levels of PLD significantly increased in MG63 cells with a roughness-dependent pattern (P<0.05P<0.05). The ALP activity and osteocalcin production, promoted by Ti surface roughness, were enhanced by the PLD activator and inhibited by the PLD blocker. It was also found that the PLD1 isoform responds to Ti surface roughness and regulates selectively the ALP activity. These observations strongly suggest that PLD1 mediates the cellular signaling of and modulates osteoblast differentiation induced by Ti surface roughness in MG63 osteoblast-like cell.

Keywords
Ti surface roughness; Osteoblast differentiation; Alkaline phosphatase activity; Osteocalcin production; Phospholipase DALP, Alkaline phosphatase; DAG, Diacylglycerol; 2*3-DPG, 2*3-diphosphoglycerate; PA, Phosphatidic acid; PBt, Phosphatidylbutanol; PC, P
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Activation of phospholipase D1 by surface roughness of titanium in MG63 osteoblast-like cell
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 32, November 2006, Pages 5502–5511
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us