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Short-term effects of adhesion peptides on the responses of preosteoblasts to pBMP-9

Paper ID Volume ID Publish Year Pages File Format Full-Text
11252 727 2008 12 PDF Available
Title
Short-term effects of adhesion peptides on the responses of preosteoblasts to pBMP-9
Abstract

Adhesion peptides are currently used to enhance the interactions of osteoblasts with biomaterials. However, little is known about the effects of adhesion peptides on cell responses to growth factors, especially the bone morphogenetic proteins (BMPs). We used adhesion peptides Ac-CGGNGERPRGDTYRAY-NH2 (pRGD), derived from bone sialoprotein, and Ac-CGGDGEA-NH2 (pDGEA), derived from collagen, which interact with αvβ3 and α2β1 integrins, respectively. We analyzed the effects of pRGD- and pDGEA-coated polystyrene (PS) on the responses of murine MC3T3-E1 preosteoblasts to a peptide derived from human BMP-9 (pBMP-9) in serum-free medium. After 1 h, pRGD favoured interactions with αv while pDGEA bound β1 integrin subunits. Adding pBMP-9 (400 ng/mL) increased the amount of αv integrin subunits in cell membranes on pRGD-coated PS, but had no effect on β1 integrin subunits. Only on this substratum, collagen type I mRNA was enhanced and the addition of pBMP-9 promoted the early cell differentiation, increasing their alkaline phosphatase (ALP) activity within 24 h. These cells also organized β1 integrin subunits at their focal adhesion points. Inhibiting α2β1 integrins by pDGEA pre-treatment decreased this ALP activity. It is therefore important to understand the impact of adhesion peptides on the early cell responses to growth factors in order to improve biomimetic materials.

Keywords
Adsorption; Alkaline phosphatase; BMP; Integrin; Osteoblast; Peptide
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Short-term effects of adhesion peptides on the responses of preosteoblasts to pBMP-9
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 8, March 2008, Pages 1005–1016
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us